In order to investigate the effect on the expression of PD-1/PD-L1 and the proportion of CD3+CD4+, CD3+CD8+ T lymphocyte in spleen lymphocytes of mice treated with Chinese herbal compound, the extract of Chinese herbal compound is obtained by traditional boiling method.The mice are given intragastric Tote Bag administration once a day with extract of Chinese herbal compound at low, medium and high concentrations.The mice are sacrificed by neck dislocation at the end of experiment time and the spleen is freshly isolated from mice.The expression of PD-1/PD-L1 and the changes of lymphocyte subtypes are detected by Real-time PCR and flow cytometry.
The results show that the expression of PD-1 on splenic lymphocytes Tank decreases significantly and the ratio of CD3+CD4+ and CD3+CD8+ T lymphocytes increases significantly with the increase of the concentration of Chinese herbal compound, but the expression of PD-L1 does not change significantly.These results suggest that the Chinese herbal compound can be used in anti-tumor immunotherapy mediated by inhibiting the expression of PD-1 in lymphocytes, activating immune helper cells and effector cells.